Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Chem-Impex International, Inc. 2-Morpholinoethanesulfonic acid | 4432-31-9 | MFCD00006181 | 1KG
2-Morpholinoethanesulfonic acid, 4432-31-9, MFCD00006181, 1KG
Boston Bioproducts Inc MES Buffer (0.5 M, pH 5.5) - 250ml
MES Buffer [2- (N-morpholino) ethanesulfonic acid] is a zwitterionic Good's buffer used in biological and biochemical research. It maintains a stable pH within the range of 5.5 to 7.0 and is highly soluble in water. MES is commonly used as a running buffer for bis-tris gels, chromatography, and in protein purification. It is valuable for enzymatic reactions, and acts as a non-coordinating buffer in metal ion chemistry. It has minimal UV absorbance and is stable in various applications.
United States Biological Corporation TRIS HCL BUFFER 1M PH7.5,500ML
Tris is one of the most widely used buffers in molecular biology and cell culture due to its low toxicity, stability and buffering capacity. Purity: ≥99.9% Appearance: White, crystalline powder pH (1M): 10.5-11.5 pKa: 8.0-8.4 Absorbance (260nm): ≤0.06 Absorbance (280nm): ≤0.05 Absorbance (290nm): ≤0.2 Solubility (1M): Colorless, clear, complete Loss on Drying: ≤0.2% Melting Point: 168-172°C Heavy Metals (Pb): ≤0.0001% Arsenic: ≤0.0001% Calcium: ≤0.0001% Copper: ≤0.0001% Iron: ≤0.0005% Magnesium: ≤0.0001% Insoluble Matter: ≤0.005% Residue on Ignition: ≤0.1% RNase: DNase: Protease: Meets specifications.
FUJIFILM BIOSCIENCES INC HEPES Buffer Solution 1M
Buffer to maintain pH between 7.2 and 7.6. Suitable for use in cell cultures outside of a CO2 incubator.
Cell Signaling Technology Tris Buffered Saline (TBS-10X) 1 liter
Tris Buffered Saline (TBS-10X) 1 liter
Scytek Laboratories ScyTek Phosphate Buffered Saline (25x), 7.6 pH, 1000ml
ScyTek Phosphate Buffered Saline (25x) pH of 7.6 is an optimal formulation of pH stabilizers and salts designed to effectively remove excess material from the microtiter plate wells without disrupting the ELISA binding reaction. By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal. Our Wash buffers do not contain hazardous preservatives such as Azide or Mercury that may interfere with antibody-antigen binding interactions. For your convenience wash buffer is offered in a wide variety of formulations to meet the needs of your specific ELISA application. This product can be customized to meet the specific needs of your assay. Inquire about custom vialing, labeling, kit assembly and drop shipping.