Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Alkali Scientific TRIS, Amino Buffer, Enzyme Grade, 5 Kilograms
TRIS Amino (Enzyme Grade) buffer is a high-purity solution designed specifically for enzyme-related applications in molecular biology and biochemistry. This 5kg package provides an ample supply of this reagent, which is widely used for preparing enzyme buffers in assays such as enzyme-linked immunosorbent assays (ELISA), protein assays, and PCR. The TRIS Amino buffer maintains stable pH conditions critical for optimal enzyme activity, and its enzyme-grade quality ensures minimal impurities that could interfere with biochemical reactions. This buffer is ideal for use in experiments where precise pH control is required for enzymatic reactions, as well as for preparing buffers for enzyme activity studies and protein purification.
eMolecules 354587-72-7 | tert-Butyl 6-(hydroxymethyl)-1h-indole-1-carboxylate | Combi-Blocks | MFCD19103503 | 247.294 | C14H17NO3 | 97.000 | CC(C)(C)OC(=O)n1ccc2ccc(CO)cc12 | 1g | 267192141
tert-Butyl 6-(hydroxymethyl)-1h-indole-1-carboxylate | Combi-Blocks | 354587-72-7 | MFCD19103503 | 247.294 | C14H17NO3 | 97.000 | CC(C)(C)OC(=O)n1ccc2ccc(CO)cc12 | 1g | 267192141
LAB BIOREAGENTS LowBinding VI Buffer 250ml
Lab Bioreagents LowBindings (I, II, III, IV, V, VI, VII) are newly developed assay buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays. The LowBindings helps to reduce the non-specific bindings, negative effects of interfering molecules, and cross-reactivities of antibodies and proteins from biomatrix. Therefore, LowBindings exhibit the low matrix interference and high signal-to-noise, by which the assay sensitivity and specificity have been significantly improved. As many factors are involved in immunoassays, there is no single assay buffer that is ideal for each assay. LowBindings (I, II, III, IV, V, VI, VII) are a variety of buffers based on different formulations.
TARGETMOL CHEMICALS INC NVP-TAE 684 10MG
Also available in 2 mg 5 mg 25 mg 50 mg 100 mg 1 mL 10 mM (in DMSO) and bulk. Please contact Fisher for quotes. NVP-TAE 684 (TAE684) is a excellently effective and specific ALK inhibitor(IC50=3 nM). purity: 99%
Bioworld TTTRIS-TRICINE) BUFFER 1
TT (TRIS-TRICINE) BUFFER, 10X SOLUTION: Tris-Tricine Tris-Tricine (TT) Buffer has been developed for both non-denaturing and denaturing electrophoresis of proteins. TT Buffer offers several advantages over more conventionally used protei...
![eMolecules 1214727-57-7 | TRANS-TERT-BUTYL (3-(AMINOMETHYL)CYCLOBUTYL)CARBAMATE | AstaTech | MFCD22123684 | 200.282 | C10H20N2O2 | 95.000 | CC(C)(C)OC(=O)N[C@H]1C[C@H](CN)C1 | 1g | 449729861](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000165855.jpg-250.jpg)
eMolecules 1214727-57-7 | TRANS-TERT-BUTYL (3-(AMINOMETHYL)CYCLOBUTYL)CARBAMATE | AstaTech | MFCD22123684 | 200.282 | C10H20N2O2 | 95.000 | CC(C)(C)OC(=O)N[C@H]1C[C@H](CN)C1 | 1g | 449729861
TRANS-TERT-BUTYL (3-(AMINOMETHYL)CYCLOBUTYL)CARBAMATE | AstaTech | 1214727-57-7 | MFCD22123684 | 200.282 | C10H20N2O2 | 95.000 | CC(C)(C)OC(=O)N[C@H]1C[C@H](CN)C1 | 1g | 449729861
Pfanstiehl Inc Tris Hydrochloride (Tris HCl) High Purity, Low Endotoxin, Low Metals (T-154) 5kg GMP CAS: 1185-53-1 Injectable Excipient Grade
Pfanstiehl Tris HCL, High Purity, Low Endotoxin, Low Metals, is a highly purified buffer component that has been specifically designed and formulated to remove impurities such as endotoxin, trace metals, and nitrosamines. Manufactured in the USA under ICH-Q7 cGMP conditions, Pfanstiehl Tris HCl meets parenteral (injectable) excipient grade standards, making it ideal for pharmaceutical, biologic, and vaccine bioprocessing and formulation. cGMP , pharmaceutical grade .
Bioworld Tricine-SDS Sample Buffer, Reducing (2X), 50 mL
Product DescriptionTricine-SDS is a sample buffer under reducing conditions for protein analysis. It has a concentration of 2X and contains 900 mM Tris-HCl with a pH of 8.45, 8% SDS, 20% glycerol, 0.01% Coomassie blue, 0.01% phenol red, and 4% β-mercaptoethanol.The tricine system requires SDS in sample and running buffers for best results. Tricine-SDS PAGE is commonly used to separate proteins that range in molecular weight between 1 – 100 kDa but is preferred for the resolution of proteins smaller than 30 kDa.
Alkali Scientific SSC Buffer 20X Powder, 2635.6 Grams of Powder, Makes 10 Liters of 20X Solution
SSC Buffer 20X Powder (2635.6 Grams) is an ultra-concentrated solution designed for DNA hybridization applications in molecular biology. This large volume of powder allows labs to prepare up to 10 liters of 20X SSC solution, ideal for high-throughput labs or large-scale experiments. SSC Buffer is critical in hybridization protocols, where high ionic strength is needed to support stable binding of nucleic acid probes to their target sequences. It is commonly used in Southern and Northern blotting, in situ hybridization, and other DNA or RNA detection techniques. The 10-liter yield ensures that large-scale or long-term projects are well-supported with this essential reagent.
Boston Bioproducts Inc M9 Minimal Salts Solution - 2x1L
M9 Minimal Salts Solution - 2x1L (For Research Use and Further Manufacturing Only)
Alkali Scientific Saline 0.85% 20mL, 10/Pk
Saline 0.85% is a sterile isotonic sodium chloride solution widely used in microbiology and laboratory testing. The concentration closely matches the osmotic pressure of bacterial and mammalian cells, helping maintain cellular integrity during handling and dilution. Unlike distilled water, which can cause cells to burst or shrink, this balanced saline preserves cell viability and structure. Because the solution contains no nutrients or buffering components, it provides an inert environment that allows microorganisms to remain stable without stimulating growth. The 20mL tube format offers a convenient volume for washing microbial cells, preparing suspensions, or diluting cultures prior to analysis in microbiological testing and quality control procedures.
Sigma Aldrich Fine Chemicals Biosciences Trizma(R) hydrochloride BioPerformance Certified, suitable for cell culture, >=99.0% (titration) | 1185-53-1 | MFCD00012590 | 100G
Trizma(R) hydrochloride BioPerformance Certified, suitable for cell culture, >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 157.6 | 1185-53-1 | MFCD00012590 | 100G